Isolation of Mesophyll Cells from Sedum telephium Leaves
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چکیده
منابع مشابه
Isolation of Mesophyll Cells and Bundle Sheath Cells from Digitaria sanguinalis ( L . ) Scop . Leaves and a Scanning Microscopy Study
A technique is described for the separation of mesophyll and bundle sheath cells from Digitaria sanguinalis leaves and evidence for separation is given with light and scanning electron micrographs. Gentle grinding of fully differentiated leaves in a mortar releases mesophyll cells which are isolated on nylon nets by filtration. More extensive grinding of the remaining tissue yields bundle sheat...
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A procedure is described for preparing intact mitochondria from leaves of Sedum praealtum D.C., a plant showing Crassulacean acid metabolism. These mitochondria oxidized malate, pyruvate, alpha-ketoglutarate, succinate, NADH, NADPH, and isocitrate with good respiratory control and ADP/O ratios better than those observed in mitochondria from other photosynthetic tissues.Malate oxidation was very...
متن کاملSeparation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies.
Mesophyll protoplasts and bundle sheath strands of maize (Zea mays L.) leaves have been isolated by enzymatic digestion with cellulase. Mesophyll protoplasts, enzymatically released from maize leaf segments, were further purified by use of a polyethylene glycol-dextran liquid-liquid two phase system. Bundle sheath strands released from the leaf segments were isolated using filtration techniques...
متن کاملIsolation of Mesophyll Cells and Bundle Sheath Cells from Digitaria sanguinalis (L.) Scop. Leaves and a Scanning Microscopy Study of the Internal Leaf Cell Morphology.
A technique is described for the separation of mesophyll and bundle sheath cells from Digitaria sanguinalis leaves and evidence for separation is given with light and scanning electron micrographs. Gentle grinding of fully differentiated leaves in a mortar releases mesophyll cells which are isolated on nylon nets by filtration. More extensive grinding of the remaining tissue yields bundle sheat...
متن کاملIsolation and Separation of Epidermal and Mesophyll Protoplasts from Rye Primary Leaves — Tissue-Specific Characteristics of Secondary Phenolic Product Accumulation
We have developed a technique for the large-scale isolation of epidermal and m esophyll proto plasts, as well as the vascular strands, o f rye primary leaf blades. Separation o f the two types of protoplasts has been successful only from leaves harvested at the end of a 13-h light period, when chloroplasts were enriched in starch. The occurrence o f different flavonoid compounds, and amounts, ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1973
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.51.1.97